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The dilution calculator is a useful tool which allows you to calculate how to dilute a stock solution of known concentration. Enter C_{1}, C_{2} & V_{2} to calculate V_{1}.

The dilution calculator is based on the following equation:

Concentration_{(start)}x Volume_{(start)}= Concentration_{(final)}x Volume_{(final)}

This equation is commonly abbreviated as:C_{1}V_{1}= C_{2}V_{2}

What volume of a given 10 mM stock solution is required to make 20ml of a 50 μM solution?

Using the equation C_{1}V_{1} = C_{2}V_{2}, where C_{1}=10 mM, C_{2}=50 μM, V_{2}=10 ml and V_{1} is the unknown:

- Enter 10 into the Concentration (start) box and select the correct unit (millimolar)
- Enter 50 into the Concentration (final) box and select the correct unit (micromolar)
- Enter 20 into the Volume (final) box and select the correct unit (milliliter)
- Press calculate
- The answer of 100 microliter (0.1 ml) appears in the Volume (start) box